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J. Chromatogr. A, 1257, 89–97. , and Moks, T. (1994) Single-step recovery of a secreted recombinant protein by expanded bed adsorption. Biotechnology, 12, 285. R. (1994) Novel affinity separation based on perfluorocarbon emulsions: use of a perfluorocarbon affinity emulsion for the direct extraction of glucose-6phosphate dehydrogenase from homogenized bakers’ yeast. J. Chromatogr. A, 659, 275–287. A. (1995) Development of an expanded bed technique for an affinity purification of G6PDH from unclarified yeast cell homogenates.

For this case, the selection of flow rate in section IV is constrained both by the upward movement of the net flux of salt and the downward movement of the net flux of myoglobin to the raffinate port. 05 M NaCl, in order to increase myoglobin ion exchange amount and promote the downward movement of myoglobin in section IV to raffinate port. However, with such a low salt concentration formed in sections III and IV, the other contaminants probably adsorb to the anion exchangers. 5 or 9, in order that a relatively high salt concentration can be used in sections III and IV to prevent the other contaminants from being adsorbed on Q-Sepharose FF resin.

Bioseparation, 8, 93–97. , and Lali, A. (2000) Enhance performance of expanded bed chromatography on rigid superporous adsorbent matrix. J. Chromatogr. A, 867, 113–130. , and Lali, A. (2001) Effect of adsorbent porosity on performance of expanded bed chromatography of proteins. Biotechnol. , 17, 522–529. -D. and Sun, Y. (2001) Nd-Fe-B alloy-densified agarose gel for expanded bed adsorption of proteins. J. Chromatogr. A, 943, 63–75. J. (2002) Fluidization and dispersion behavior of small high density pellicular expanded bed adsorbents.

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