By Christopher D. Richardson
Baculovirus Expression Protocols deals either commercial and university-based researchers a accomplished comilation of the newest baculovirus suggestions besides step by step directions and time-saving options. The contributors-leading specialists within the field-present the various expression plasmids at present in use, advisor the reader during the strategy of producing and choosing recombinant virus, and describe particular examples of recombinant protein creation and purification. The emphasis is on substitute and less complicated screening options for the choice of recombinant baculovirus. The e-book additionally surveys many of the insect mobile strains at present suitable with the baculovirus approach. Highlights comprise construction of recombinant virus utilizing linearized DNA and vectors that comprise a b-galactosidase indicator and a whole checklist of expression vectors at present to be had. particular descriptions for the scale-up of protein construction utilizing spinner flasks, bioreactors, and bug larvae also are incorporated. Baculovirus Expression Protocols bargains either commercial and university-based researchers a great choice of reproducible, step by step laboratory protocols. it is going to instantly develop into quintessential for someone operating with baculoviruses and their software within the expression of recombinant proteins in insect cells.
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Extra info for Baculovirus Expression Protocols
3 kbp), used asthe starting point for constructing the vectors. The large flanking regions either side of the polyhedrin gene locus also ensured that homologous recombination took place in virus-infected cells. 5 kbp), Baculovirus Transfer Vectors 31 which may facilitate easier insertion of foreign DNA into the plasmid. Variable amounts of the 3’-coding region from the polyhedrin gene have been conserved and the expression vector generally contains the polyhedrin termination and polyadenylation signal (Table 1).
1990) Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acid Res. l&5667-5672 Lopez+ erber, msk, and Yossee brJ 12 Kitts, P. A. and Possee, R. D. (1993). A method for producing recombinant baculovirus expression vectors at high frequency. Biorechniques 14,810-817. 13. Smith, G. , Ericson, B. , and Summers, M. D. (1985) Modification and secretion of human interleukin 2 in insect cells by a baculovirus expression vector. Proc. Nut. Acad. Sci.
20,97-104. 10. , Voncken, J. , van Lier, F L. , and Vlak, J. M. (1991) Detection and analysis of Autographa californica nuclear polyhedrosis virus mutants with defective interfering properties. Virology 183,739-746 11. , Ayres, M. , and Possee, R. D. (1990) Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acid Res. l&5667-5672 Lopez+ erber, msk, and Yossee brJ 12 Kitts, P. A. and Possee, R. D. (1993). A method for producing recombinant baculovirus expression vectors at high frequency.